Synthesis and Growth of HAp Crystals

Synthesis and Growth of HAp Crystals Results and discussions Synthesis of HAp/Chitosan/Dopamine films Two types of HAp-chitosan composite films were prepared: HAp-chitosan films with and without dopamine (Figure 1). The weight ratio between HAp and chitosan in the films was fixed to 50 wt% since the HAp content in human bones is about 50-70wt% in dry weight28-30. In comparison with the 50 wt% HAp-chitosan, 70 wt% HAp-chitosan films containing dopamine were also generated, but this composite was too brittle to be used as hard tissue substitute. HAp-chitosan films were successfully casted and the films were slightly yellow in color. Dopamine containing HAp-chitosan films were also casted and the color was changed to dark brown. The color alteration of the films was due to the oxidation of dopamine that formed phenolic tanning compounds19,31. Based on the previous studies, wet precipitation methods were carried out to synthesize a nano-HAp in the presence of chitosan and its derivatives13,16. Chitosan strongly interacted with HAp and regulated the anisotropic growth of crystalline HA. I n addition, it was revealed that dopamine can also facilitate the growth of the HAp crystals along the c-axis21. Therefore, the synthesis and growth of HAp crystals in the presence of both chitosan and dopamine were examined by FTIR, XRD and SEM before conducting the mechanical analysis and water uptake analysis. FTIR analysis and Wide-angle X-Ray Diffraction FTIR spectra of the composite films are shown in Figure 1. Generally, hydroxyl and phosphate bands of HAp and characteristic peaks of chitosan are present in all of the composites FTIR spectra regardless of dopamine addition. More specifically, FTIR spectra of the composites showed the identity bands of HAp; stretching and bending vibration modes from the phosphate groups () were identified at absorption bands of 898 1095 cm-1 and 477 660 cm-1 respectively. The combination band of hydroxyl (O-H) bending and libration modes was observed at 630 cm-1. Furthermore, observed band at about 3600 cm-1 indicated the O-H stretching. On the other hand, several typical chitosan absorption peaks of 1150, 1375, 1640 cm-1and 2900 cm-1were observed in the chitosan containing composites. Peak at 1150 cm-1 was indicating glucosamine unit. The peak observed at 1640 cm-1 represents amide I (C=O) and anti-symmetric NH3 deformation. The band appearing at 1599 cm-1is attributed to amide II bands. Both am ide I and amide II show a hydrogen bond between –NH2 and –OH of HAp. Peak at 2900 cm-1 represent the –CH2 backbone. In the dopamine containing composite, the polyphenolic content resembled by phenolic COH peak that was discerned at 1260 cm-1. Moreover, both of aromatic C=C and COO bands were also observed at 1600-1650 cm-1 FTIR is an appropriate technique to observe the composite constituent interaction. It measures the frequencies at which chemical functional groups absorb as the result of the sample’s chemical interaction. In this regards, the appearance of glucosamine unit band at 1150 cm-1 that overlap with the stretching vibrations bands of HAp indicated that HAp crystals were formed on the chitosan molecules through certain interaction. In addition, chitosan interaction with ions by means of phosphorylation were also identified by the emergence shoulder at 1220 cm-1 and an increased at 1064 cm-1 absorption peaks of the chitosan containing composites spectra12. Chitosan has great affinity to react with ions without pH dependent13. This interaction makes chitosan tend to undergo phosphorylation in acid, basic and neutral solutions. In the presence of the phosphorilated groups, chitosan can strongly bind with intermediate form of HAp, amorphous calcium phosphate (ACP), and impose constrain ts of ACP subsequently lead to crystalline HAp formation13. Furthermore, chelation of calcium ions by phosphate functionalities may also induce the formation of crystalline HAp. The XRD spectra of the samples with or without dopamine also support the existence of HAp crystalline phase in the composite films. Most peaks in the XRD spectra of the samples could be indexed to the known HAp structure (Ca10(PO4)6) with characteristic peaks at 2ÃŽ ¸ regions of 26Â º, 29Â º, 32-34Â º, 40Â º, 46-54Â º, which are consistent with HAp phase (JCPDF #09-0432), confirming that the phase was formed in all samples13,32. However, the crystallinity of HAp in the chitosan-HAp composite films was lower than 100% HAp powder due to the presence of chitosan. The broad peak around 20Â º is an indicative peak for chitosan in the composite film regardless of dopamine addition13. Interestingly, some evidence which support anisotropic growth of Hap in the presence of dopamine were shown in the XRD spectra. The intensities of HAP diffractions relating to (002), (300) and (211) peaks (at 2ÃŽ ¸ of 26 Â º, 32 Â º, 33 Â º respectively) were measured. The ratio of the measured diffra ction intensity of c-axis (002) to another direction was used to determine the orientation degree. The XRD results after Gaussian Fit indicates that the (002) to (300) intensity ratio of 50% HAp samples with and without dopamine was 0.17 and 0.45 respectively. This indicated the preferential orientation of the HAp growth in the c-axis was significantly increased with the presence of dopamine. Additional broad peaks (~10Â º ~15Â º) were observed in the dopamine containing film. It indicates that the addition of dopamine induced structural changes in d-spacing over 0.6-0.9 nm in the film due to dopamine-mediated crosslinking, or dopamine-mediated HAp growth. Overall, XRD spectra suggest that the aspect ratio and anisotropy increased in the dopamine containing HAp/chitosan composite. SEM, TEM analysis and Cell Test Result To examine the effect of dopamine addition on the surface morphology, the dopamine-containing films were observed under SEM and TEM (Figure 3 and 4). The figures show a presence of nanorod particles in the composites with narrow and uniform particle size distribution in all samples. In the absence of dopamine, this structure is likely formed due to phosphorylation of chitosan which bind with phosphate precursor compounds and modulate the crystallization of HAp13,16. In the presence of dopamine, the aspect ratio of HAp was increased up to ~4.7 fold compared with control HAp in the absence of chitosan nor dopamine (figure x). For a comparison, in the 50 wt% composite, the aspect ratios are 2.4 and 4.5, without and with dopamine crosslink respectively. The dopamine effect is probably because of catecholic group from the dopamine bind with Ca2+ in HAp crystals formation 21. The pKa dopamine is ~8.9, dopamine was added while the pH decreased from ~8 to 4.2 The protonated cathecholic group of dopamine are possibly involved in HAp formation and regulate the one-dimensional growth of HAp crystals. This phenomenon is well agreed as the previous experiment result that polydopamine addition on HAp provides mechanism for surface-anchored catecholamine moieties to enrich the interface with calcium ions, facilitating the formation of hydroxyapatite crystals19.The addition of dopamine not only guides the anisotropic directional growth of hydroxyapatite crystals which increased its aspect ratio, but also changed the homogeneity of the grain distribution and shape of the nanostructure (Figure 5). To study the effect of HAp content on the aspect ratio of the nanostructure, the 25% and 70 wt% HAp-chitosan film with/without dopamine was synthesized. As the results, the aspect ratio is increases with increasing of HAp weight %. The 70 wt% with dopamine containing sample showed the highest aspect ratio. (Supporting figure X). The aspect ratio is a significant property of HAp related to the absorbability and fracture toughness of the samples. Higher aspect ratio known to have better adsorbability since it is proportional to the surface area of rods3, which are beneficial for cell attachment. However, aspect ratio alone is inadequate to identify the cellular affinity of sample, as a previous study suggested that surface roughness can also play an important role33. It was also found that aspect ratio and surface roughness of the composite film have a significant effect on the cell attachment and proliferation1. We tested MC-3T3 cells (mouse pre-osteoblast cell line) proliferation on the HAp-chitosan composite surfaces. To measure the dependence of MC-3T3 cell viability and proliferation on surface materials quantitatively, WST-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt] based cell counting assay was performed. WST-8 produced yellow-colored product (formazan) when it was reduced by dehydrogenases in living cells34,35. The levels of cell viability and proliferation on the sample that contains dopamine were slightly lower than others (Figure 6) suggesting that the increasing of HAps aspect ratio is not always beneficial because the cell viability was rather decreased. Nevertheless, the number of viable cells in the sample is still increased, yet at a slightly lower rate than the other sample. This is implying that this material would probably have no strong cytotoxicity. However, in vivo testing is remains to be proved the cytotoxicit y of the dopamine-containing composites.

Reversing The Aging Process, Should We? Essay -- essays research paper

Reversing The Aging Process, Should We? In the length of time measured as human lifetime one can expect to see a full range of differing events. It is assumed that during a lifetime a person will experience every possible different emotion. If one is particularly lucky, he will bear witness to, or affect some momentous change in humanity. However is it reasonable to ask what would be experienced by someone who lived two lifetimes? Up until recently the previous question would and could only be rhetorical. There is no answer, because no one has ever lived that long. Of course that was up until now. At McGill University, nematodes (tiny organisms) have experienced five lifetimes (Kluger). Through complex scientific experiments nematodes and fruit flies have had their lifespans increased not by fractions of life times, but by multiples of lifetimes (Kruger). Mankind is using the discovery of DNA as an opportunity to play G-d by changing the aging process. Man has a natural tendency to play the role of G-d. Man has a an inherent need to affect others, be it through the vises of war, power, manipulation or politics. However man’s natural tendency to play G-d has reached it’s final manifestation. By attempting to slow down the aging process man is using himself as the ultimate canvas, to play the role of the omnipotent. Research into the process of aging began in 1961(Rose, Technology Review:64). Since then a great deal of time, money and effort have been appropriated into discovering the causes of aging, it can therefore be inferred that humanity has an almost "personal" interest in aging. Of course the culmination of discovering how we age, is discovering how to stop it. An intrinsic characteristic of Man is His obsession with superficiality. Superficiality is equated with appearance. The appearance of beauty can be equated with youth. Therein lies man’s obsession with age, ceasing to age means being eternally beautiful. As usual man’s actions are dominated by ego and self-preservation. Within the confines of youth there lies a certain fountain of power. Power which cannot be accessed once one ages. Things like physical and sexual prowess. The time of youth is often refereed to as the "prime of your life". It is therefore not difficult to understand and conceive of man’s motivation to stay young and to wish that the immediate people surr... ...m control ling microscopic chemical reactions. Man is referred to as G-d’s ultimate creation, the universe his canvas. But what happens when humans steal the canvas and decide to redecorate, would you want to recolor your Picasso? Is there any justification for living that long, does there need to be? These are not easy questions, and there not intended to be, but should scientists prove successful in their endeavors, all of these questions will have to be resolved. How can certain establishments which frown on cosmetic plastic surgery frown on the reorganization of protein strands? There is no doubt that the people in charge of those organizations would take advantage of these technologies (Rose, Melatonin,: 6). How are the two things different? There are no possible answers to these questions for now they must remain rhetorical. It is increasingly obvious that the repercussions of these technologies stretch across the board. As always the horizon of the future stretches before us, only revealing a glimpse of that which is to come. The resounding questions that will soon confront us can only be concluded with the passage of time, something apparently humanity will have a lot of.

Icu Case Study

Case Study A 45 year old male was admitted to ICU following an exploratory laparotomy which showed a ruptured appendix and peritonitis. The procedure began as a laparoscopic cholecystectomy but the initial finding was pus throughout the peritoneal cavity and a normal gallbladder. An open exploratory laparotomy where a ruptured appendix was discovered which was removed and a washout was performed. The patient had a two day history of abdominal pain prior to his admission through A&E. He had no previous medical or surgical history. The patient smoked 20/day and drank alcohol at the weekends.Once admitted to ICU, he was intubated and ventilated on bilevel ventilation and sedated with propofol and fentanyl. In theatre he received two litres of hartmanns solution as a fluid load, however in ICU was commenced on maintenance of normal saline at 100mls/hr. Feeding was ruled out on admission as it was thought that the patient would have extubated the following day. However, the patient was in ICU six days prior to extubation, therefore TPN was commenced. Noradrenaline was used for a MAP above 70mmhg rather than a fluid load. The patients clinical scenario was more in depth as outlined above.However, these are outside the scope of this case study. The medical and nursing interventions discussed in this assignment is mechanical ventilation, total parental nutrition and vasopressors. Mechanical Ventilation Bilevel ventilation is a relatively new setting. (Mireles-Cabodevila et al, 2009) The ventilator maintains a high pressure setting for the bulk of the respiratory cycle, which is followed by a release of low pressure. (Mireles-Cabodevila et al, 2009) The release to a low PEEP is the expiration phase and aids the elimination of CO?.The release periods are kept short to prevent derecruitment of alveoli and encourage spontaneous breathing. (Mireles-Cabodevilla et al, 2009) The advantages of bilevel include an increase in mean alveolar pressure with recruitment, haemodynamic and ventilatory benefits and reduced sedation requirements. (Putensen et al, 2006) Analgesia and sedation is not only used for pain relief and anxiety but for mechanical ventilation comfort. (Putensen et al, 2006) This level can reduced to aim of a Riker of 4, which a co-operative, responsive patient. Putensen et al, 2006) This reduces the need for more vasopressors to maintain a stable cardiovascular system. (Putensen et al, 2006) When first admitted the patients ventilatory settings were: FiO2 0. 4 Rate 12 HiPeep 22 LoPEEP 5 PS 10 CXR showed bibasal atelectasis/consolidation ABG – pH 7. 43 paCO? 33 paO? 74 HCO? 23 BE -0. 5 The pH is within normal limits, on the lower end, i. e. between 7. 35 – 7. 45. Therefore it is normal/alkalotic. The paCO? indicates an alkalotic range. This is used to assess the effectiveness of ventilation. (Coggon, 2008) PaO? is 74, which is low as normal range is 80-110, which shows hypoxemia.PaO? is not interpreted in the patients acid-base status but indicates O? binding to haemoglobin. (Coggon, 2008) The HCO? is normal. The next step is to match the CO? , HCO? to the pH. The CO? and pH is on the alkalotic side of normal. Therefore it shows a respiratory disturbance. (Woodruff, 2009) The next step is to see if either compensation is occurring. To do this, the interpretor must look to see if either the CO? or HCO? go in the opposite direction of the pH. In which, in the ABG above, you can clearly see that it does although the HCO? is within normal range, which means no compensation is occurring. Woodruff, 2009) The full diagnosis is uncompensated respiratory alkalosis with hypoaxemia. The patient is more than likely hyperventilating with poor gas exchange in view of the CXR. In response to this ABG result ventilatory setting were changed to: FiO2 0. 4 Rate 8 HiPEEP 22 LoPEEP 8 PS 14 ABG post setting change – pH 7. 39 paO? 103 paCO? 36 HCO? 22 The rate was changed as the patient was blowing off too much CO? with the rate of 12 plus any spontaneous breaths he was doing. The patients chest was rotten with a productive secretions and bibasal consolidation at the bases, seen in a repeat CXR.Suctioning resulted in moderate to large amounts of white sputum. The patients wife stated that he had been suffering from a cold for one week prior to admission. Therefore an increase in PEEP (Hi and Lo) was required to recruit the alveoli and aid in good gas exchange. (Dellinger at al, 2007) It is recommended that positive end expiratory pressure is set to avoid extensive lung collapse on expiration. (Dellinger et al, 2007) Maintaining pressure and spontaneous breathing resulted in an increase in arterial oxygenation and helped prevent a deterioration in pulmonary gas exchange. Putensen et al 2006) Studies have been carried out to determine whether high PEEP increases patients outcomes. These include the ALVEOLI study and the Lung Open Ventilation (LOV). These studies do not show an improvement on mortalit y with the increase in PEEP however show a decrease in days on the ventilator. (Mercat et al 2008) The patients right side was worse than the left on the CXR and auscultation. Therefore he was being positioned right side up and his back on pressure area cares. Repositioning patients not only protects the patients skin but it also improves gas exchange and decrease the risk of ventilator acquired pneumonia. Deutschmann and Neligan, 2010) Positioning the patient with the good lung down may improve paO? and aid in the drainage of secretion. (Deutschmann and Neligan, 2010) Elevating the head of the bed also aids recruitment of alveoli at the bases and again decreases the risk of ventilator acquired pneumonia. (Deutschmann and Neligan, 2010) All of the above interventions by nursing and medical staff were to improve the patients outcome and aid extubation once the patients chest improved and any other factors affecting the patients ability to self-ventilate. Total Parenteral NutritionIt is seen as appropriate time-scale of 1 – 3 days that surgical patients commence normal diets. (Braga et al, 2009) As the patient was intubated and ventilated, no feeding was commenced until day three. The main goal of nutritional support is to avoid starvation in the aim to support post-operative recovery, and maintain the patients normal body functions. (Braga et al, 2009) Malnutrition decreases patients outcomes within the critical care setting. (Artinian et al, 2006) Total parenteral nutrition (TPN) was commenced at 40mls/hr as per the ICU Dr's orders.The dietician reviewed the patient and suggested the goal rate was 81mls/hr, which the feed was slowly increased to over two days. this is because of the risk of referring syndrome. Refeeding syndrome is ‘a syndrome consisting of metabolic disturbances that occur as a result of reinstitution of patients who have been starved or malnourished. ‘ (Shils et al, 2006) The medical and nursing team must keep a close eye on the patients bloods. Although this is standard practice with all patients in ICU. Refeeding syndrome can cause a multitude of complications including, neurological, pulmonary, cardiac and hematologic. Assiotisa and Elenin, 2010) The use of the dietician greatly reduces the risk of over-feeding. (Ziegler, 2009) However, the consensus is the patients are underfed as medical teams are conservative in their approach of prescribing rates. (Faisy et al, 2009) Although the dieticians are heavily involved in the ICU that the patient in this study is, recent studies have shown that this is a good standard of care, as this helps doctors and nurses focus on early nutrition prescribed at the correct rate. (Faisy et al, 2009) The most used formulae used to predict goal rate is 25/kcal/kg ideal body weight. Braga et al, 2009) However in intubated patients, there is a fluctuating in ‘resting energy expenditure' due to the use of sedatives, analgesics and vasopressors causing confusion ove r energy given and uptake. (Faisy et al, 2009) The bag of TPN the patient had is ?. This is appropriate as patients requiring TPN need a full range of vitamins and trace elements daily. (Braga et al, 2009) Enteral nutrition is widely used in ICU due to the increased risk of TPN induced catheter-related sepsis, cost and multi-organ failure. Faisy et al 2009) However, surgical patients are less likely to receive enteral nutrition compared to medical patients. (Elke et al, 2008) Previous studies investigating critical ill abdominal surgical patients suggested that early feeding is beneficial. (Artinian et al, 2006) Nevertheless, another report suggested it did more harm than good, resulting in an increase of infections. (Artinian et al, 2006) The current recommendations, is that patients whom are expected to commence a normal intake should be started on parenteral nutrition. Singer et al, 2009) The patient who was still sedated and ventilated at this stage falls under the recommendatio ns. His bowel sounds were scant and he did not have a bowel motion since admission. The surgeons were reluctant to commence feeding with the absence of bowel sounds. This is due to the fear of a paralytic ileum as peritonitis may cause this. (McClare et al, 2009) It is now acknowledged that gentle feeding may restore gut mobility and is recommended for early management. (McClare et al, 2009) The lack of sound evidence based practice results in a need for future studies on post-operative feeding. Lownfels, 2008) While the patient is on TPN, an insulin protocol in in place to monitor blood sugar levels second hourly and adjust insulin as required. There is a high risk of hyperglycaemia due to insulin resistance when the body is under increased stress. (Braga et al, 2009) Therefore close glucose control is of benefit to the patients outcome in the ICU setting, including fewer infectious episodes and lower mortality. (Braga et al, 2009) A central line or another type of central access i s recommended for the administration of TPN, as it can irate the veins in peripheral access. Singer et al, 2009) A study performed about the reliability of central venous lines and PICC lines came to the conclusion that the number of infections was the same, nevertheless, phlebitis and thrombus occurred more frequently in the PICC lines. (Singer et al, 2009) Feeding the patient is a complicated process and a close eye on the patient is needed is pick up on complications that may occur during the feeding regime. Once the patient was extubated. , he remained drowsy for a day and a half. Ammonia levels were done and these came back high. The TPN was stopped and the patient's mental status slowly improved.The patients LFT's were normal. Vasopressors The patient is also being treated for an abdominal infection and community acquired pneumonia. Septic shock is defined as an inflammatory response syndrome with a mean arterial pressure (MAP) of 70mmhg. Other factors affecting the cardiovasc ular system include sedatives. (Ray and McKeown, 2007) Therefore the systemic infection and sedatives are contributing to the patients low blood pressure. Vasopressors and intropes are used when volume replenishment is not able to adequately increase blood pressure or with this patient fluid resuscitation is not considered (as he already had 2000mls of luid intra-operatively). (Morrell et al, 2009) Sufficient fluid loading is recommended prior to vasopressor use to try and stabilise the patient with septic shock. (Dellinger et al, 2009) Vasopressor therapy is used to maintain tissue perfusion in the event of critical illness. (Dellinger et al, 2007) Perfusion is reliant on pressure and control of vascular beds are inevitably lost when mean arterial pressure falls below a certain point. (Dellinger et al, 2007) Within the unit the patients are treated with norepinephrine (noradrenaline), which is the drug of choice when treating shock induced hypotension.This is because norepinephrine is a potent drug and is very effective at treating hypotension compared to other drugs. (Dellinger et al, 2007) Norepinephrine is an ? -adrenergic agonist and has some ? -adrenergic effects. (Urden et al, 2006) Noradrenaline is naturally released by nerve cells, producing the fight or flight response within the body. (Urden et al, 2006) This would normally produce an increased heart rate, increased blood pressure, dilated pupils, dilate air passage in the lungs and narrowing of blood vessels in non-essential organs, which aids the body in coping under stressful situations. Urden et al, 2006) The alpha receptors are found in muscle tissue, therefore by stimulating these receptors, noradrenaline causes the muscles to contract resulting in narrowing of blood vessels. (Urden et al, 2006) This means that an increase in MAP and systemic vascular resistance with little alteration in heart rate and volume output. (Morrell et al, 2009) Intravenous infusions of noradrenaline at low doses has been reported to increase blood pressure, urinary output and creatinine clearance, resulting in an aid to overall decreasing vasopressor therapy. Morrell et al, 2009) However, as with most drugs, side-effects of high doses of vasopressors, which include headache, bradycardia, hypertension, and inadequate blood flow leading to low levels of oxygen in extremities. (MIMMS, 2011) Studies involving small doses of vasopressin show an improvement in blood pressure over a small period of time. (Russell et al, 2008) The titration of noradrenaline is the nurses responsibility once the doctors order the aim MAP. (Brown and Edwards, 2008) An important part of nursing care is comprehensively assessing the patient receiving vasopressors. Brown and Edwards, 2008) These must include, urinary output, consciousness (if able), colour, temperature, pulses of the extremities, heart rate, blood pressure, signs and symptoms of myocardial schema. (Brown and Edwards, 2008) Titration of the drug is based on current observations. An important aspect to consider when using vasopressors, is to treat the cause of the shock. (Dellinger et al, 2009) This aids in recovery the the decreasing usage of invasive procedures. The use of noradrenaline on the patients improves his oxygenation and outcomes through this acute illness.The main objective is to improve oxygenation and noradrenaline has an impact on oxygenation by increasing preload, leading to an increased cardiac output. It also has an impact on cardiac contractility, which increases the force ejection thus allowing the heart to over come any increase in afterload caused by the vasoconstriction. (Urden et al, 2006) The increase consumption of oxygen, oxygen consumption increases. (Brown and Edwards, 2008)This is due to the hypermetabolic rate the critical ill patient is under.Caring for critically ill patients requires an in-depth knowledge on bodily systems and functions. Within the ICU environment, hierarchy of nurses which range from junior to senior. This exists to aid growth within the junior members of staff, as continuing education is important. As the above case study shows, numerous problems need to be addressed throughout the care of the patient. As only three issues have been addressed, this only illustrates a small insight into the scope of care the patient received. References Mercat, A. t al, (2008) Positive End-Expiratory Pressure settings in Adults with Acute Lung Injury and Acute Respiratory Distress Syndrome: A Randomised Controlled Trial. JAMA, 6: 646-655 McClare, S. A. , et al (2009) Guidelines for the provision and assessment of nutrition support therapy in the adult critical ill patient: Society of Critical Care Medicine and American Society for Parenteral and Enteral Nutrition, JPEN, 33: 277-316 Lownfels, A. B. (2008) Recovery after abdominal surgery: Is enteral feeding preferable? A best evidence review. http://www. medscape. org/newarticale/568983 Shils, M.E. , et al (2006) Modern nutritio n in health and disease, 10th edition, Lippincott Williams and Wilkins, Baltimore Assiotisa, A. , Elenin, H. (2010) Implications of refeeding syndrome in post-operative total parenteral nutrition. http://www. grandrounds-e-med. com/articles/gr100013. htm Deutschmann, C. S. , Neligan, P. J. (2010) Evidence-Based Practice of Critical Care, Saunders, Philadelphia Coggon, J. (2008) Arterial blood gas analysis: Understanding ABG reports. Nursing Times; 104: 18, 28-29 Woodruff, D. (2009) 6 Easy Steps to ABG Analysis. http://www. Ed4nurses. com Faisy, C. et al (2009) Impact of energy deficit calculated by a predictive method on outcome in medical patients requiring prolonged acute mechanical ventilation, British Journal of Nutrition, 101, 1079-1087 Singer, P. , et al (2009) ESPEN Guidelines on Parenteral Nutrition: Intensive Care, Clinical Nutrition, 28, 387-400 Braga, M. , et al (2009) ESPEN Guidelines on Parenteral Nutrition: Surgery, Clinical Nutrition, 28, 378-386 Antinian, V. , et al (2006) Effects of early enteral feeding on the outcome of critically ill mechanically ventilated medical patients, CHEST, 129, 960-967 Elke, G. et al (2008) Current practice in nutritional support and its association with mortality in septic patients – Results from a national, prospective, multicenter study, Critical Care Medicine, 36, 1762-1767 Putensen, C. , et al (2006) The impact os spontaneous breathing during mechanical ventilation, Current Opinion in Critical Care, 12, 13-18 Mireles-Cabodevila, E. , et al (2009) Alternative modes of ventilation: A review for the hospitalise, Cleveland Clinic Journal of Medicine, 76, 417-430 Morrell, M. R. , et al, (2009) The Management of Severe Sepsis and Septic Shock. Infec Dis Clin N Am, 23, 485-501 Ray, D. C. McKeown, D. W. (2007) Effect of induction agent on vasopressor and steroid use, and outcome in patients with septic shock. Critical Care, http://www. ccforum. com/content/11/3/R56 Russell, J. A. , et al, (2008) Vasopressin vee rs Norepinephrine Infusion in Patients with Septic Shock, The New England Journal of Medicine, 358, 877-887 Brown, D. , Edwards, H. (2008) Lewis's medical-surgical nursing: Assessment and Management of Clinical Problems, 2nd edition, Mosby, China Urden, L. D. , et al, (2006) Thelan's Critical care Nursing: Diagnosis and Management, 5th edition, Mosby, China MIMMS (2011) http://www. mimms. com. au

Claude Monet and Impressionism Essay - 2256 Words

Claude Monet and Impressionism Claude Monet was born in Paris on the 14th November, 1840. When he was five years old, he moved to the port town of Le Havre. For much of his childhood, Monet was considered by both his teachers and his parents to be undisciplined and, therefore, unlikely to make a success of his life. Enforcing this impression, Monet showed no interest in inheriting his fathers wholesale grocery. The only subject which seemed to spark any interest in the child was painting. He developed a decent reputation in school for the caricatures he was fond of creating. By the age of fifteen, he was receiving commission for his work. It was at Le Havre that Monet met the painter Eugene Boudin. While Boudins own paintings†¦show more content†¦The landscapes and colors of Algeria presented an entirely different perspective of the world, one which was to inspire him for many years to come. Theoretically, Monet should have remained in Algeria for seven years, but his time there was curtailed by the contraction of typhoid. The artists aunt, Madame Lecadre, intervened and bought Monet out of the army. Her only condition: that Monet return to Paris and make a serious attempt at completing a formal artistic tuition course. Despite these provisions, Monet did not enroll in lÉcole des Artistes . It was a renowned institution, but one filled with the traditionalists that Monet was so determined to contradict. Instead, he joined the studio of the Swiss-born Charles Gleyre. Gleyre was a successful Salon painter but he was neither a professor at the École nor was he a member of the Acadà ©mie . Remembering his own poverty as a student artist, Gleyre charged very little , only 10 francs for models and the studio. This leniency attracted a large number of artists. The student body, such as it existed, was extremely diverse: young, old; rich, poor; good, bad, etc. Among them all, however, Monet was to meet three very cl ose and influential friends: Frà ©dà ©ric Bazille, Auguste Renoir and Alfred SisleyThis subcategory of Gleyres students was representative of the studios diverse constitution. While all three of these painters were talented, they came from very different social backgrounds. Noticeably ,Show MoreRelatedClaude Monet and Impressionism1107 Words   |  4 PagesWhen you talk about impressionism, you will always connect it with Oscar Claude Monet but there were many impressionist artists beside Claude Monet. But in this composition we will talk more about Claude Monet because he was the founder of Impressionism in the world. Oscar Claude Monet was the founder of impressionism. He is a famous french painter whose work gave a name to the art movement impressionism. Claude Monet’s famous quote is â€Å"merely think here is a little square of blue, here an oblongRead MoreThe Characteristics Of Claude Monet As An Impressionism809 Words   |  4 PagesClaude Monet is best known for his work as an impressionist, not only was he able to capture art in a whole new meaning but he influenced and led the way to a whole new technique. The impressionist movement was a group of artist who attempted to capture the emotion of their scene. They would paint their paintings in a non-realistic way, in the beginning Monet and his companions were ridiculed for such departure but in time were praised. Monet loved painting outdoors he loved capturing the sceneRead MoreClaude Manet – Impressionism – 19Th . Oscar-Claude Monet1449 Words   |  6 PagesClaude Manet – Impressionism – 19th Oscar-Claude Monet was a founder of French Impressionist painting, and the most consistent and productive expert of the movement s philosophy of communicating one s observations before nature particularly applied to plein-air landscape painting. The expression Impressionism is from the title of his piece Impression, soleil levant (Impression, Sunrise), which was shown in 1874 in the first of the independent presentations mounted by Monet and his partners asRead MoreClaude Monet s Cataracts : Blurring The Lines Through Impressionism Essay2162 Words   |  9 PagesCataracts: Blurring the Lines Through Impressionism Isabella Kalish Art 323 Modernism Kimberly Anderson December 9, 2016 Claude Monet is perhaps one of the most well known artists in recent times. His career spanned nearly seven decades and his work inspired a new generation of artists. As impressive as this sounds, we may still ask what was the reasoning behind his success? It is no secret that towards the end of his life and career,Monet was losing his eyesight as a resultRead MoreClaude Monet Theory1047 Words   |  5 Pagesthe ideal, bold blue. Claude Monet and Georges Pierre Seurat not only understood this key philosophy but based their entire collections of artwork on it. From using splashes of colorful brush strokes to thousands of little dots, Monet’s and Seurat’s style and approaches to art, stemming from color theory influences, revolutionized their individual artistic time period. Claude Monet’s approach to landscapes vastly differed from previous generations’ of artists. The work of Monet excluded ultrafine detailsRead MoreAutumn on the Seine, Argenteuil by Claude Monet Essay963 Words   |  4 Pagesthe Seine, Argenteuil. This oil on canvas painting can be found in the High Museum of Art. Claude Monet, the artist of this piece painted this in 1873, right as the Impressionism Movement was beginning. Monet played the important role of one of the founders of the Impressionism Movement with his works like Autumn on the Seine, Argenteuil. Autumn on the Seine, Argenteuil is from a series of paintings that Monet did while in Argenteuil. In the artwork Autumn on the Seine, Argenteuil, the lighting usedRead MoreClaude Monet Essay1193 Words   |  5 Pagesobserving, criticizing and appreciating art. Claude Monet’s piece titled Sunrise (Marine) illustrates the daylight in the industrial port of Le Havre of the north coast, France. This piece was made in March or April of 1873. The piece’s present location is the J. Paul Getty Museum, west pavilion, gallery w204. The medium is oil on canvas and is next to another piece made by Monet called The Portal of Rouen Cathedral in the morning light. Claude Monet was part of the impressionist movement that changesRead MoreA Group Of French Artists In Paris, Of Which Included Claude1641 Words   |  7 Pagesartists in Paris, of which included Claude Monet and Berthe Morisot, worked together in their academic painting, while rejecting Salons and becoming independent from the Acadà ©mie. They created a self-supporting group rather than relying on state-sanctioned institutions (Stokstad 987). Impressionists challenged the way paintings were viewed, and critics often viewed their paintings as unfinished. As a result, they unknowingly started the movement of Impressionism, and the movement changed the way artRead MoreImpressionism as a Avant-garde Movement970 Words   |  4 Pages analyses the aesthetic and ideological underpinnings of the Modernist artwork, Impression, Sunrise of Claude Monet. The artwork and Impressionism is considered to be a visual articulation of the avant-garde and the latter statement is explained. References to the writings of Charles Harrison, Clement Greenberg and Wilhelm Worringer is used to theorise the aesthetics of modernity. â€Æ' 2. IMPRESSIONISM AS MODERN ART Modernism is the heartbeat of culture, or as Clement Greenberg (1992:754) states, modernismRead More Claude Monet Essay1280 Words   |  6 PagesClaude Monet Claude Monet made the art community address a revolutionary type of art called impressionism. In a style not previously before painted, impressionism captured a scene by using bright colors with lots of light and different shades to create the illusion of a glance. The traditional method of working in a studio was discarded and the impressionist artists carried any needed supplies with them into the countryside and painted the complete work outside. The manufacture of portable tin